USDA Utilizes Atlas System for Rapid Detection of High Salmonella Levels in Beef Lymph Nodes without Enrichment

Posted by Erin Dreyling, PhD on May 27, 2015 5:05:00 PM

Atlas in Action: As technology advances, the development of new tools and new applications will continuebifscoto provide the beef industry practical, implementable solutions. Here is another example of describing a unique application using the Atlas System.


Researcher Mick Bosilevac, PhD from the USDA Agricultural Research Service has developed a novel application for detection of Salmonella at enumerable levels in beef lymph nodes utilizing the Atlas Salmonella Generation II (SG2) assay without enrichment. Dr. Bosilevac screened 1,038 subiliac lymph nodes with the Atlas SG2 assay without enrichment. For each sample, a paired culture and enumeration result, if applicable, was obtained post-enrichment. Dr. Bosilevac’s work found that 73 percent of samples with enumerable Salmonella was detected by the Atlas SG2 assay without enrichment. Regression analysis estimated that the Atlas SG2 assay had a 0.5 CFU/gram Limit of Detection. Screening of lymph node samples without enrichment on the Atlas system, allows for rapid Salmonella detection within 3.5 hours. While, common PCR methods required a 24 hour enrichment, and culture with enumeration require 4 days for completion, Dr. Bosilevac’s research demonstrates that the Atlas SG2 assay offers a rapid and cost-effective solution for identification of Salmonella at enumerable levels in beef lymph nodes.

For further information regarding Dr. Bosilevac’s project, please download his research poster presented at the March 2015 BIFSCO Meeting.

BosilevacBIFSCo2015PosterHandout_thumb

Download Poster

 

 

Topics: Salmonella Pathogen Detection