Atlas in Action: Researchers Utilize Atlas System to Identify Salmonella from Texas Beef Cattle Feedlots In Order to Design Food Safety Interventions for Beef.

Posted by Jim Dolan on May 5, 2015 1:33:00 PM

Roka is committed to partnering with the Food Industry to enable the progress of food safety science to meet both current and future demands. Below is an example of how the Atlas System can be incorporated for not only just routine testing but in the development of applicable new tools and strategies to enable these types of practical solutions from concept to market.

Research recently presented by a team of scientists in the Department of Animal Science at Texas A&M University (College Station, TX) reported the presence and identification of differing serotypes of Salmonella enterica recovered from Texas beef cattle feedlots. The team included Matthew Taylor, Ph.D. (Project Leader), Jason Gill, Ph.D., Ashley N. Arnold, Ph.D., Kerri B. Gehring, Ph.D., Jeffrey W. Savell, Ph.D., and Miss Yicheng Xie, Graduate Research Assistant.

The presence and carriage of Salmonella serovars, particularly antimicrobial resistant salmonellae, in cattle at harvest may compromise the safety of fresh beef. The researchers sought to identify the serotypes of Salmonella recovered from these feedlots as well as identify the presence of Salmonella-infecting bacteriophages (phages) from sampled Texas feedlots. Three feedlots in south Texas that had been previously characterized for Salmonella prevalence were visited and samples collected from dropped feces, feed, drinking water, and soil in cattle pens. Following pre-enrichment in lactose broth, salmonellae were selectively enriched and plated. A representative subset of presumptive Salmonella isolates was then confirmed biochemically and subsequently serotyped. Roka Bioscience, Inc., partnered with Texas A&M to offer required supplies and reagents for researchers to perform nucleic acid amplification-based detection of Salmonella from the lactose broth pre-enrichment fluid samples using the Atlas® System located at the Texas A&M Center for Food Safety (College Station, TX).

Roka_01From amongst all samples, 85.0% of recovered salmonellae were collected from a single feedlot, with the remainder from a second feedlot. From a set of 38 isolates representative of Salmonella-positive samples, S. Anatum was recovered from all sample types. Salmonella Montevideo, Muenchen, Altona, Kralingen, and Kentucky were also recovered. In a comparison of culture-based detection of Salmonella versus the Atlas System, testing results yielded only one disagreement, where the single discrepant result was between an Atlas negative and a culture positive.

These data were presented at the 2015 Beef Industry Safety Summit meeting in Dallas, TX, where researchers presented results of Salmonella detection and identification of feedlots, as well as antimicrobial resistance phenotypes of recovered salmonellae. Additionally, one refereed manuscript is under final development for submission and publication, with other manuscripts planned for development and publication and presentation. Research was sponsored by The Beef Checkoff and by the Texas Beef Council, and all reagents and supplies required for completion of Atlas System testing were generously gifted to researchers by Roka Bioscience. Research findings are being used by Texas A&M’s team to continue to discover practical solutions for protecting beef safety, such as in this case the identification of Salmonella-infecting bacteriophages that may be utilized to prevent Salmonella transmission to consumers on beef products.

If you want to learn more about the great work Texas A&M University is doing, check out the following links:

Dr. Gill Lab page 
Texas A&M Department of Animal Science
AgriLife Communications
Texas A&M Center for Phage Technology
Texas A&M Center for Food Safety 

And if you want to learn more about Roka Bioscience's Beef Testing Solutions?  Download our E-bookAchieving Faster Turnaround Time on E. coli O157:H7 and STEC Results in Beef Trim, Ground and FTP.



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Topics: Salmonella Pathogen Detection, Atlas in Action